forensic science geek
1. Blue “glow” appears to be chemiluminescence.
2. Blue chemiluminescence is produced when hemoglobin in blood catalyzes the decomposition of a peroxide to form free oxygen. The free oxygen reacts with luminol. At the end of the reaction cascade, the luminol has been transformed to an excited state molecule. The excited state molecule returns to the ground state accompanied by the emission of light.
3. Limitations include production of “false positives” (dependent upon application method) with several different materials including metals and oxidizers (cleaning agents, etc.) and dilution of small quantities of blood, which may affect subsequent DNA analysis.
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